Anti-Sox9

Description_x000D_ General description_x000D_ Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal._x000D_ Specificity_x000D_ Recognizes Sox9._x000D_ Immunogen_x000D_ Epitope: C-terminal_x000D_ KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Sox9._x000D_ Application_x000D_ Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB._x000D_ Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Sox9 in murine embryonic bone and adult cartilage tissue sections._x000D_ Western Blotting Analysis: An 1:500 dilution from a representative lot detected Sox9 in human PC3 prostate cancer cells and HepG2 hepatocytes._x000D_ Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at target chromatin sites by ChIP using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243)._x000D_ Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at the Bmi promoter in Z/sox9tg but not in wild-type control mouse embryonic fibroblasts/MEFs (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315)._x000D_ ChIP-sequencing (ChIP-seq) Analysis: A representative lot detected Sox9-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243)._x000D_ Immunofluorescence Analysis: A representative lot detected the accumulation of Sox9-positive oval cells by fluorescent immunohistochemistry staining of paraffin-embedded liver sections from transgenic mice treated with diethylnitrosamine to induce conditional liver HNF4a knockout (Saha, S.K., et al. (2014). Nature. 513(7516):110-114)._x000D_ Immunofluorescence Analysis: Representative lots detected Sox9 immunoreactivity in paraffin-embedded mouse embryo sections by fluorescent immunohistochemistry (Carrasco, M., et al. (2012). J. Clin. Invest. 122(10):3504-3515; Sylva, M., et al. (2011). PLoS One. 6(8):e22616)._x000D_ Immunofluorescence Analysis: Representative lots immunostained Müller glial cells in frozen mouse and chicken retina sections by fluorescent immunohistochemistry staining of (Muranishi, Y., and Furukawa, T. (2012). J. Biomed. Biotechnol. 2012:973140; Fischer, A.J., et al. (2011). Neuroscience. 178:250-260)._x000D_ Immunocytochemistry Analysis: A representative lot detected the stem cell marker Sox9 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441)._x000D_ Immunohistochemistry Analysis: A representative lot immunostained the supporting cells (Sertoli) of the seminiferous tubules by immunohistochemistry staining of paraffin-embedded mouse testis sections (O′Shaughnessy, P.J., et al. (2012). PLoS One. 7(4):e35136)._x000D_ Immunohistochemistry Analysis: A representative lot detected Sox9 immunoreactivity in various formalin-fixed, paraffin-embedded human tumor tissue sections (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315)._x000D_ Western Blotting Analysis: A representative lot detected the stem cell marker Sox9 in E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441)._x000D_ Western Blotting Analysis: A representative lot detected upregulated Sox9 expression level in human colorectal cancer cell lines, HCT116, DLD1, and SW620 (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315)._x000D_ Research Category_x000D_ Epigenetics & Nuclear Function_x000D_ Research Sub Category_x000D_ Transcription Factors_x000D_ Target description_x000D_ 56-65 kDa_x000D_ Physical form_x000D_ ImmunoAffinity Purified_x000D_ Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide_x000D_ Storage and Stability_x000D_ Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in HepG2 cell lysate._x000D_ Western Blotting Analysis: An 1:2000 dilution of this antibody detected Sox9 in HepG2 cell lysate._x000D_ Linkage_x000D_ Replaces: AB5809_x000D_ Analysis Note_x000D_ Control_x000D_ Embryonic tissue, Adult chondrocytes._x000D_ Other Notes_x000D_ Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.